| Applications |
Suzanne Epstein that reacts with the gp120 of HIV-1. In ELISA the antibody reacts strongly with rgp120 IIIB. In immunofluorescence assays, it binds weakly to cell surface expressed gp160 and also shows weak cross-reactivity by ELISA with gp120 MN (an isolate not used during immunization). The antibody does not bind to the gp120 synthetic peptides that were used to map antibody reactivity and was not able to cross block any other antibodies in the panel. |
| Comments |
Animals were sequentially immunized with recombinant gp120 (rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB, SF2, and Z6). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. 55-36 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1. In ELISA the antibody reacts strongly with rgp120 IIIB. In immunofluorescence assays, it binds weakly to cell surface expressed gp160 and also shows weak cross-reactivity by ELISA with gp120 MN (an isolate not used during immunization). The antibody does not bind to the gp120 synthetic peptides that were used to map antibody reactivity and was not able to cross block any other antibodies in the panel. |
| References |
Reeves JP, et al. Mouse monoclonal antibodies to human immunodeficiency virus glycoprotein 120 generated by repeated immunization with glycoprotein 120 from a single isolate, or by sequential immunization with glycoprotein 120 from three isolates. Hybridoma 14: 235-242, 1995. PubMed: 7590785
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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