| 產品名稱 |
58-S |
| 商品貨號 |
B163790 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is type specific for HSV-1 but does not neutralize the virus. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against herpes simplex virus type 1 (HSV-1) immediate early protein ICP 4 (175000 dalton) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against herpes simplex virus type 1 (HSV-1) immediate early protein ICP 4 (175000 dalton) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with HSV-1. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody is type specific for HSV-1 but does not neutralize the virus. |
| Complete Growth Medium |
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%. |
| Subculturing |
Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions |
Atmosphere: 5% CO2 in air recommended |
| Isotype |
IgG2a |
| Name of Depositor |
The United States of America |
| U.S. Patent Number |
|
| References |
Showalter SD, et al. Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4. Infect. Immun. 34: 684-692, 1981. PubMed: 6277788
Zweig M, et al. Monoclonal antibodies to herpes simplex virus type 1 polypeptides. US Patent 4,572,896 dated Feb 25 1986
|
