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6D8MB4

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編號:B163805

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產品名稱	6D8MB4
商品貨號	B163805
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody is specific for the gp50 envelope glycoprotein of PRV.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against pseudorabies virus (PRV)
Cellular Products	immunoglobulin; monoclonal antibody; against pseudorabies virus (PRV)
Tumorigenic	Yes
Effects	Yes, in BALB/c mice
Comments	Animals were immunized with pseudorabies virus (IA strain 62-26). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the gp50 envelope glycoprotein of PRV.
Complete Growth Medium	Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵ cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL. Medium Renewal: Two to three times weekly.
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG2b
Name of Depositor	CA Whetstone
Deposited As	mouse (B cell); mouse (myeloma)
References	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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