| 產品名稱 |
71A7 |
| 商品貨號 |
B163809 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the Con A molecule at a site distinct from the sugar binding site. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3U1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against concanavalin A (Con A) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against concanavalin A (Con A) |
| Comments |
Animals were immunized with C.AL-20 (a T cell line) cells that had been incubated for 48 hours with 5 mcg/ml of Con A. Spleen cells were fused with P3U1 myeloma cells. The antibody reacts with the Con A molecule at a site distinct from the sugar binding site. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
FL Owen |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Spurll GM, Owen FL. Development of a cell line secreting monoclonal antibody specific for concanavalin A and use of that antibody as an affinity immunosorbent for tissue culture media used to support long-term T cell growth. J. Immunol. Methods 39: 335-341, 1980. PubMed: 6161967
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