| 產品名稱 |
76-5-28 |
| 商品貨號 |
B163819 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody cross-reacts with and shows variable cytotoxic activity against other peripheral blood leukocyte populations. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against pig macrophage and T cell; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against pig macrophage and T cell; cytotoxic |
| Comments |
Animals were immunized with thymocytes from dd miniature swine. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is cytotoxic for all dd haplotype macrophages. The antibody cross-reacts with and shows variable cytotoxic activity against other peripheral blood leukocyte populations. Tested and found negative for ectromelia virus (mousepox). THIS CELL LINE IS UNSTABLE AND MUST BE RECLONED AS SOON AS POSSIBLE AFTER THAWING. |
| Complete Growth Medium |
Culture Medium: Modified Dulbecco's medium (see below), 90%; fetal bovine
serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM; kappa light chain |
| Name of Depositor |
JR Lunney |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Pescovitz MD, et al. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J. Immunol. 133: 368-375, 1984. PubMed: 6609988
|
