| 產品名稱 |
7A9 |
| 商品貨號 |
B163823 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody binds to IL-1 activated human endothelial cells, but not to resting endothelial cells or IL-1 treated mononuclear leukocytes, fibroblasts or keratinocytes. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against interleukin 1 (IL-1) activated human endothelial cells |
| Cellular Products |
immunoglobulin; monoclonal antibody; against interleukin 1 (IL-1) activated human endothelial cells |
| Comments |
Animals were immunized with IL-1 activated human umbilical vein endothelial cells. Spleen cells were fused with mouse myeloma cells. The antibody binds to IL-1 activated human endothelial cells, but not to resting endothelial cells or IL-1 treated mononuclear leukocytes, fibroblasts or keratinocytes. It precipitates an antigen consisting of a 100000 dalton polypeptide (90000 daltons under reducing conditions). The antibody totally blocks binding of granulocytes and partially blocks monocyte binding to IL-1 stimulated endothelial cells. |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640 with 0.016 mM thymidine, 0.1 mM hypoxanthine,
85%; fetal bovine serum, 15%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
Otsuka Pharmaceuticals Co., Ltd. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Newman W, Wilson DO. Monoclonal antibodies directed to IL-1 activated endothelial cells and medicaments employing the monoclonal antibodies. US Patent 5,011,778 dated Apr 30 1991
|
