| 產品名稱 |
7C8 |
| 商品貨號 |
B163825 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody can be used to detect and purify proteins produced in an expression system using a a vector containing the sequence coding for the polypeptide. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. The antibody can be used to detect and purify proteins produced in an expression system using a a vector containing the sequence coding for the polypeptide. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a synthetic polypeptide (NH2-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Gly-Pro-Lys-Lys-Gly-COOH) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a synthetic polypeptide (NH2-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Gly-Pro-Lys-Lys-Gly-COOH) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with the above synthetic polypeptide. Spleen cells were fused with NS-1 myeloma cells. The antibody can be used to detect and purify proteins produced in an expression system using a a vector containing the sequence coding for the polypeptide. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 5 X 10 exp5 viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM |
| Name of Depositor |
Immunex Corporation |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Hopp TP, et al. Synthesis of protein with an identification peptide. US Patent 4,703,004 dated Oct 27 1987
Hopp TP, et al. Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same. US Patent 4,782,137 dated Nov 1 1988
|
