| 產品名稱 |
7F2-6D4-8E7 |
| 商品貨號 |
B163831 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
FIV |
| Applications |
The antibody reacts with an astrovirus group specific antigen. It reacts with all five known serotypes of astrovirus, and with the Marin County agent. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified astrovirus type 2. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against astrovirus group antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against astrovirus group antigen |
| Comments |
Animals were immunized with purified astrovirus type 2. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody reacts with an astrovirus group specific antigen. It reacts with all five known serotypes of astrovirus, and with the Marin County agent. |
| Complete Growth Medium |
Depositor's culture medium: RPMI 1640, 80%; hybridoma cloning factor, 5%; fetal
bovine serum, 15%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
Univ. Mass. Med. Cntr. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Herrmann JE, Blacklow NR. Antibodies specific for the group antigen of astroviruses. US Patent 5,556,746 dated Sep 17 1996
|
