| 產品名稱 |
83-12-5 |
| 商品貨號 |
B163838 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with Lyt 2.2. |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse Lyt 2.2 (CD8 alpha 2.2) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse Lyt 2.2 (CD8 alpha 2.2) |
| Comments |
Animals were immunized with the BM10-5.6 cloned CTL (cytotoxic T lymphocyte) line. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with Lyt 2.2. The antibody will fix complement. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
JA Bluestone |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Leo O, et al. Identification of monoclonal antibodies specific for the T cell receptor complex by Fc receptor-mediated CTL lysis. J. Immunol. 137: 3874-3880, 1986. PubMed: 3097135
|
