| 產品名稱 |
86D [127.5-9] |
| 商品貨號 |
B163839 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the sheep gamma/delta TCR and precipitates molecules of 42000 and 36000 daltons. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the sheep (ovine) gamma/delta T cell receptor |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the sheep (ovine) gamma/delta T cell receptor |
| Comments |
Animals were immunized with double negative (DN) sheep T cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the sheep gamma/delta TCR and precipitates molecules of 42000 and 36000 daltons. |
| Complete Growth Medium |
CULTURE MEDIUM: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
CR Mackay |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Mackay CR, et al. Gamma/delta T cells express a unique surface molecule appearing late during thymic development. Eur. J. Immunol. 19: 1477-1483, 1989. PubMed: 2528463
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