| 產品名稱 |
9AE10 |
| 商品貨號 |
B163852 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes. It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic) |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with rabbit spleen cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes. It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain. The antigen is a glycoprotein with an apparent molecular weight of 25000 daltons and may be a rabbit analogue of the Thy-1 antigen. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
RPMI 1640 medium, 85%; horse serum, 10%; fetal bovine serum, 5%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
KL Knight |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
McNicholas JM, et al. Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody. Mol. Immunol. 18: 815-822, 1981. PubMed: 7040945
McNicholas JM, et al. Functional studies of rabbit T lymphocytes. Immunology 43: 635-641, 1981. PubMed: 6456220
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