| 產品名稱 |
A11.1 M |
| 商品貨號 |
B163864 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with Epstein-Barr virus transformed human B cells (HLA phenotype A11,A24,B27,B44,Bw4,Cw2). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA A11, A24; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA A11, A24; cytotoxic |
| Comments |
Animals were immunized with Epstein-Barr virus transformed human B cells (HLA phenotype A11,A24,B27,B44,Bw4,Cw2). Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for all HLA A11 positive cells and HLA A24 cells at an appropriate titer. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml. |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG3 |
| Name of Depositor |
F Grumet |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Foung SK, et al. A monoclonal antibody against HLA-A11 and A-24. Hum. Immunol. 15: 316-319, 1986. PubMed: 2420768
Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637
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