| 產品名稱 |
A-204 [A204] |
| 商品貨號 |
B163868 |
| Organism |
Homo sapiens, human |
| Tissue |
muscle |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
rhabdomyosarcoma |
| Age |
1 year |
| Gender |
female |
| Karyotype |
diploidy and tetraploidy |
| Clinical Data |
female |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice; forms small cell malignant tumor consistent with embryonal rhabdomyosarcoma; also tumorigenic in newborn mice treated with anti thymocyte serum |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10,13 D13S317: 11,12 D16S539: 11,12 D5S818: 12 D7S820: 8,10 THO1: 8,9.3 TPOX: 8,9 vWA: 15,17 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor |
DJ Giard |
| Deposited As |
Homo sapiens |
| References |
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
|
| Cross References |
Nucleotide (GenBank) :
M17560
Simian SRV-1 type D retrovirus (infectious clone) long terminal repeat RNA.
Nucleotide (GenBank) :
M17561
Simian SRV-1 type D retrovirus (noninfectious clone) long terminal repeat RNA.
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