| 產品名稱 |
A2E11 |
| 商品貨號 |
B163871 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). The antibody is highly specific for aldosterone and does not cross-react significantly with other steroids. |
| Derivation |
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against aldosterone |
| Cellular Products |
immunoglobulin; monoclonal antibody; against aldosterone |
| Comments |
Animals were immunized with a BSA conjugate of the 3-O carboxylmethyl oxime of aldosterone. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). The antibody is highly specific for aldosterone and does not cross-react significantly with other steroids. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 1 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol and 1 mM L-glutamine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
CE Gomez-Sanchez |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Gomez-Sanchez CE, et al. The production of monoclonal antibodies against aldosterone. Steroids 49: 581-587, 1987. PubMed: 3453564
|
