| 產品名稱 |
A6 |
| 商品貨號 |
B163888 |
| Organism |
Xenopus laevis, frog, South African clawed |
| Tissue |
kidney |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
adult |
| Gender |
male |
| Clinical Data |
male |
| Virus Resistance |
poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia; pseudorabies |
| Complete Growth Medium |
NCTC 109 medium, 75%; distilled water, 15%; fetal bovine serum, 10%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 26°C |
| Name of Depositor |
KA Rafferty |
| Deposited As |
Xenopus laevis |
| References |
Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969.
Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803
|
