| 產品名稱 |
AF6-120.1.2 |
| 商品貨號 |
B163916 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with cells bearing the I-A of the b, k and u H-2 haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against I-A b |
| Cellular Products |
immunoglobulin; monoclonal antibody; against I-A b |
| Comments |
Animals were immunized with spleen cells from C57BL/10J mice. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with cells bearing the I-A of the b, k and u H-2 haplotypes. The antibody does not react with I-A of the d, f, q, r or s H-2 haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Interval: Maintain cultures at a cell concentraton between 5 X 10(4) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgG2a |
| Name of Depositor |
AM Stall |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J. Immunol. Methods 50: R85-R112, 1982. PubMed: 7047643
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