| 產品名稱 | AHH-1 |
|---|---|
| 商品貨號 | B163921 |
| Organism | Homo sapiens, human |
| Tissue | peripheral blood |
| Cell Type | B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 2 [Cells contain Herpesvirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 33 years |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products. They are are sensitive to killing by benzo[a]pyrene. |
| Storage Conditions | liquid nitrogen vapor phase |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype | near diploid |
| Images |  |
| Clinical Data | male Caucasian 33 years |
| Comments | The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products. They are are sensitive to killing by benzo[a]pyrene. The cells carry an endogenous Epstein-Barr virus (EBV) genome and are EBNA positive. |
| Complete Growth Medium | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; horse serum, 10%
|
| Subculturing | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: 2 to 3 times per week
Note: The cells grow as floating aggregates of large cell clusters. |
| Cryopreservation | Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X,Y CSF1PO: 10 D13S317: 11,13 D16S539: 10 D5S818: 12,13 D7S820: 10,11 THO1: 6,9.3 TPOX: 8,9 vWA: 18,19 |
| Population Doubling Time | 16 to 19 hrs |
| Name of Depositor | Massachusetts Institute of Technology |
| Deposited As | Homo sapiens |
| U.S. Patent Number | |
| References | Crespi CL, Thilly WG. Mutation assays involving blood cells that metabolize toxic substances. US Patent 4,532,204 dated Jul 30 1985 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
