Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain
Strain:? BALB/c (B cell); BALB/c (myeloma)
Applications
The antibody reacts with 2134P fimbriae of enterotoxigenic Escherichia coli.
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against 2134P fimbriae of enterotoxigenic Escherichia coli
Cellular Products
immunoglobulin; monoclonal antibody; against 2134P fimbriae of enterotoxigenic Escherichia coli
Comments
Animals were immunized with a crude extract containing the 2134P antigen prepaed from Escherichia coli, strain 2134 bacteria.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody reacts with 2134P fimbriae of enterotoxigenic Escherichia coli.
The antibody blocks the binding of 2134P+ bacteria to intestinal epithelial cells.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 2 mM L-glutamine, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Isotype
IgG1
Name of Depositor
National Animal Disease Center, ARS, USDA
References
Dean-Nystrom EA, et al. A monoclonal antibody identifies 2134P fimbriae as adhesins on enterotoxigenic Escherichia coli isolated from postweaning pigs. Vet. Microbiol. 37: 101-114, 1993. PubMed: 7905220
