| 產品名稱 |
AN3 CA |
| 商品貨號 |
B163942 |
| Organism |
Homo sapiens, human |
| Tissue |
uterus; endometrium |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
adenocarcinoma |
| Age |
55 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
55 years Caucasian female |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice Yes, in the cheek pouch of cortisone treated hamsters |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 sq. cm flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X
CSF1PO: 13*
D13S317: 12,14
D16S539: 10,14
D5S818: 11,14
D7S820: 7*,10,7.1
THO1: 10,9.3*
TPOX: 8,10
vWA: 14,20
*Note: This cell line has historically exhibited instability at CSF1PO 13, D7S820 7, and THO1 9.3 |
| Isoenzymes |
AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1-2 |
| Name of Depositor |
CJ Dawe |
| Deposited As |
Homo sapiens |
| References |
Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
The cells produce undifferentiated malignant tumors.
at low frequency (22%)
C. J. Dawe and associates derived this cell line from a metastatic lesion in the lymph node of a patient with endometrial carcinoma alerted to the condition by onset of the malignant disorder acanthosis nigricans.
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