| Derivation |
This cell line derived from ovarian tissues of the moth, Antheraea eucalypti, by Grace in 1962, constituted the first true line of arthropod cells established in cell culture. Because of the difficulty and expense in obtaining significant volumes of lepidopteran hemolymph, the Antheraea cells were adapted to hemolymph-free culture medium by Yunker, Vaughn
and Cory . The cell line was grown in Grace's insect tissue culture medium supplemented with 10% FBS (heat-inactivated), 10% whole chicken egg ultrafiltrate and 1% bovine plasma albumin (fraction V). The cells grow predominantly in suspension although some cells adhere to the vessel walls. Yunker and Cory found the adapted Antheraea cells were able to support the growth of a number of arboviruses. |
| Comments |
This cell line derived from ovarian tissues of the moth, Antheraea eucalypti, by Grace in 1962, constituted the first true line of arthropod cells established in cell culture. Because of the difficulty and expense in obtaining significant volumes of lepidopteran hemolymph, the Antheraea cells were adapted to hemolymph-free culture medium by Yunker, Vaughn
and Cory . The cell line was grown in Grace's insect tissue culture medium supplemented with 10% FBS (heat-inactivated), 10% whole chicken egg ultrafiltrate and 1% bovine plasma albumin (fraction V). The cells grow predominantly in suspension although some cells adhere to the vessel walls. Yunker and Cory found the adapted Antheraea cells were able to support the growth of a number of arboviruses. |
| References |
. . Nature 195: 1, 1962.
Yunker CE, et al. Adaptation of an insect cell line (Grace's Antheraea cells) to medium free of insect hemolymph. Science 155: 1565-1566, 1967. PubMed: 6020481
Yunker CE, Cory J. Infection of Grace's Antheraea cells with Arboviruses. Am. J. Trop. Med. Hyg. 17: 889-893, 1968. PubMed: 5726135
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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