| Comments |
Animals were immunized with 60S spliceosomes purified by gel filtration. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with a 35000 dalton protein present in nuclear extracts. On Western blots, the antibody detects a doublet at about 35000 daltons. It can be used to deplete nuclear extracts of splicing activity, to detect mammalian SC35 by Western blot, to precipitate purified spliceosomes and to study mRNA splicing in vitro. |
| References |
Fu XD, Maniatis T. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343: 437-441, 1990. PubMed: 2137203
Spector DL, et al. Associations between distinct pre-mRNA splicing components and the cell nucleus. EMBO J. 10: 3467-3581, 1991. PubMed: 1833187
Fu XD, Maniatis T. The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site. Proc. Natl. Acad. Sci. USA 89: 1725-1729, 1992. PubMed: 1531875
Fu XD, Maniatis T. Isolation of a complementary DNA that encodes the mammalian splicing factor SC35. Science 256: 535-538, 1992. PubMed: 1373910
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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