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AP.6

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編號(hào):B163950

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產(chǎn)品名稱(chēng)	AP.6
商品貨號(hào)	B163950
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody reacts with the alpha chain of the AP-2 protein of all mammalian species tested. It is useful for immunofluorescence and immunoprecipitation assays, but tends to give spurious bands in Western blots.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against AP-2 adaptor protein of clathrin coated vesicles
Cellular Products	immunoglobulin; monoclonal antibody; against AP-2 adaptor protein of clathrin coated vesicles
Comments	Animals were immunized with adaptor protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the alpha chain of the AP-2 protein of all mammalian species tested. It is useful for immunofluorescence and immunoprecipitation assays, but tends to give spurious bands in Western blots.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing	Cultures can be established by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 2 x 10⁵ viable cells/mL. Maintain cell density between 10⁵ and 10⁶ viable cells/mL. Medium Renewal: Two to three times weekly
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG1
Name of Depositor	FM Brodsky
Deposited As	mouse (B cell); mouse (myeloma)
References	Chin DJ, et al. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc. Natl. Acad. Sci. USA 86: 9289-9293, 1989. PubMed: 2574457 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

序號(hào)	文章標(biāo)題	操作
1	ATCC細(xì)胞運(yùn)輸和處理	查看詳情
2	其他海外品牌代理	查看詳情
3	ATCC代理	查看詳情
4	購(gòu)買(mǎi)須知	查看詳情
5	訂購(gòu)流程	查看詳情

梅經(jīng)理	17280875617	1438578920
胡經(jīng)理	13345964880	2438244627
周經(jīng)理	17757487661	1296385441
于經(jīng)理	18067160830	2088210172
沈經(jīng)理	19548299266	2662369050
李經(jīng)理	13626845108	972239479