| 產品名稱 | AtT-20/D16v-F2 |
|---|---|
| 商品貨號 | B163963 |
| Organism | Mus musculus, mouse |
| Tissue | pituitary, anterior |
| Product Format | frozen |
| Morphology | small rounded cells |
| Culture Properties | loosely adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | tumor |
| Strain | LAF1 |
| Applications | This clone has been used successfully by Moore et al. for several DNA mediated transfection studies relating to endocrine and exocrine secretory pathways.
The cells are readily transfected using a standard calcium phosphate protocol. |
| Storage Conditions | liquid nitrogen vaor phase |
| Images |  |
| Genes Expressed | adrenocorticotropic hormone (ACTH) |
| Cellular Products | adrenocorticotropic hormone (ACTH) |
| Comments | The F2 subclone of AtT-20 (see ATCC CCL-89) was developed by B. Gumbiner.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Cells grow in patches and pile up. Cultures do not become confluent.
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Three times weekly |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vaor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor | RB Kelly |
| Deposited As | Mus musculus |
| References | Gumbiner B, Kelly RB. Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells. Cell 28: 51-59, 1982. PubMed: 6279313 Moore HP, et al. Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation. Cell 35: 531-538, 1983. PubMed: 6317196 Burgess TL, et al. The exocrine protein trypsinogen is targeted into the secretory granules of an endocrine cell line: studies by gene transfer. J. Cell Biol. 101: 639-645, 1985. PubMed: 2991303 Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 Stefana B, et al. Leukemia inhibitory factor induces differentiation of pituitary corticotroph function: an immuno-neuroendocrine phenotypic switch. Proc. Natl. Acad. Sci. USA 93: 12502-12506, 1996. PubMed: 8901611 |
| Cross References | Nucleotide (GenBank) : U79773 Mus musculus NNP-1 (NNP-1) mRNA, complete cds. Nucleotide (GenBank) : U79774 Mus musculus NNP-1 var (NNP-1) mRNA, complete cds. Nucleotide (GenBank) : NM_010925 Mus musculus novel nuclear protein 1 (Nnp1), mRNA. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
