| 產品名稱 |
B2-1 |
| 商品貨號 |
B163976 |
| Organism |
Mus musculus, mouse |
| Tissue |
embryo |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
embryo |
| Strain |
BALB/c |
| Applications |
B2-1 cells are thymidine kinase negative (TK-) and are resistant to IUdR. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Tumorigenic |
No |
| Effects |
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments |
B2-1 cells are thymidine kinase negative (TK-) and are resistant to IUdR. |
| Complete Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with:
0.05 mg/ml 5-iodo-2'-deoxyuridine
10% heat-inactivated fetal bovine serum
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Remove IUdR for one passage before using. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
The United States of America |
| U.S. Patent Number |
|
| References |
Hampar B, Showalter SD. Non-transformed thymidine kinaseless cell line and its use for testing tumorigenic potential of genes. US Patent 4,465,769 dated Aug 14 1984
Hampar B, et al. Herpes simplex virus (type 1) thymidine kinase gene does not transform cells morphologically. Proc. Natl. Acad. Sci. USA 78: 2616-2619, 1981. PubMed: 6264486
|
