| 產品名稱 |
B27M2 |
| 商品貨號 |
B163979 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic |
| Comments |
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2). Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for a subpopulation of HLA B27 positive cells and for all HLA Bw47 positive cells at an appropriate titer. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Modified RPMI 1640 medium (see below) with 50 uM
2-mercaptoethanol, 90%; fetal bovine serum, 10%.
RPMI 1640 100 mL
L-Glutamine (100x) 1 mL
Solution FM 1 mL
Non-essential amino acids (100x) 1 mL
Fetal Bovine Serum 12 mL
Solution FM (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 100 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 450 mg (41 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
F Grumet |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637
|
