| 產品名稱 |
B6.2 |
| 商品貨號 |
B163984 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma,sarcoma |
| Applications |
The antibody reacts with extracts of both metastases, and with live BT-20 (ATCC HTB-19), MCF7 (ATCC HTB-22) and ZR-75-1 (ATCC CRL-1500) mammary carcinoma cells. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human mammary tumor cells |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human mammary tumor cells |
| Comments |
Animals were immunized with membrane enriched fractions of extracts from either of two human breast tumor metastases to the liver (Met 1 and Met 2). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with extracts of both metastases, and with live BT-20 (ATCC HTB-19), MCF7 (ATCC HTB-22) and ZR-75-1 (ATCC CRL-1500) mammary carcinoma cells. The antibody does not react with liver extracts or a variety of live normal and sarcoma cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended Medium Renewal: 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO 5% (v/v) Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: 5% CO2 in air recommended |
| Isotype |
IgG1 |
| Name of Depositor |
NIH |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Colcher D, et al. A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc. Natl. Acad. Sci. USA 78: 3199-3203, 1981. PubMed: 6789331
Colcher D, et al. Process for producing monoclonal antibodies reactive with human breast cancer. US Patent 4,522,918 dated Jun 11 1985
|
