| 產品名稱 |
B8-24-3 |
| 商品貨號 |
B163988 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Lymphoma |
| Applications |
The antibody reacts with all cells bearing H-2 of the b haplotype and cross-reacts with the H-2 p and pa haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against H-2 b |
| Cellular Products |
immunoglobulin; monoclonal antibody; against H-2 b |
| Comments |
Animals were immunized with EL4 lymphoma cells mixed with Bordetella pertussis bacteria. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with all cells bearing H-2 of the b haplotype and cross-reacts with the H-2 p and pa haplotypes. It does not react with the d, f, j, k, q, s, v, u, w1 and W15 haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
G Kohler |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Kohler G, et al. Characterization of a monoclonal anti-H-2Kb antibody. Immune Syst. 2: 202-208, 1981.
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