| 產品名稱 |
BBM.1 |
| 商品貨號 |
B164006 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human Beta-2 microglobulin; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human Beta-2 microglobulin; cytotoxic |
| Comments |
Animals were immunized with MOLT-4 cells (ATCC CRL-1582, a human T cell line). Spleen cells were fused with P3X63Ag8 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2b |
| Antigenic Determinant |
Microglobulin, beta-2, human |
| Name of Depositor |
P Parham |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Brodsky FM, et al. Characterization of a monoclonal anti-B2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur. J. Immunol. 9: 536-545, 1979. PubMed: 91522
Parham P, et al. Arginine 45 is a major part of the antigenic determinant of human beta2-microglobulin recognized by mouse monoclonal antibody BBM.1. J. Biol. Chem. 258: 6179-6186, 1983. PubMed: 6189821
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