| 產品名稱 |
BC-1 |
| 商品貨號 |
B164007 |
| Organism |
Homo sapiens, human |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 CELLS CONTAIN HERPESVIRUS
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Age |
46 years |
| Gender |
male |
| Applications |
Transfection host.
It is also a practical source of KSHV DNA to be used as a positive control for screening tests. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
BC-1 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles.
The cells were derived from an acquired immunodeficiency syndrome (AIDS) related body cavity based lymphoma presenting as a primary lymphomatous effusion in the peritoneal cavity. |
| Antigen Expression |
CD30 +; CD45 +; EMA + (epithelial membrane antigen) |
| Comments |
The cells contain two viral genomes: Epstein-Barr virus (EBV) and Kaposi sarcoma associated herpesvirus (KSHV, provisionally designed HHV-8).
KSHV is a recently identified and largely uncharacterized virus.
The BC-1 cell line allows in vitro culture of KSHV viral genomes. |
| Complete Growth Medium |
RPMI 1640 medium, 80%; fetal bovine serum, 20%
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 105 cells/mL and maintain between 5 x 105 and 2 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,11 D13S317: 8 D16S539: 12,13 D5S818: 11,12 D7S820: 11 THO1: 9.3 TPOX: 8,9 vWA: 16,20 |
| Population Doubling Time |
48 to 72 hrs |
| Name of Depositor |
E Cesarman |
| Deposited As |
Homo sapiens |
| References |
Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568
Cesarman E, et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86: 2708-2714, 1995. PubMed: 7670109
Chadburn A, et al. CD30 (Ki-1) positive anaplastic large cell lymphomas in individuals infected with the human immunodeficiency virus. Cancer 72: 3078-3090, 1993. PubMed: 8221575
Cesarman E, et al. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS- related body-cavity-based lymphomas. N. Engl. J. Med. 332: 1186-1191, 1995. PubMed: 7700311
|
