| 產品名稱 |
BE29G1 |
| 商品貨號 |
B164021 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antiboy is useful for studies of inflammation, lymphocyte differentiation and lymphocyte activation. It can be used for purification of ICAM-1 and for staining of cell surface ICAM-1 by flow cytometry. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Clinical Data |
The antiboy is useful for studies of inflammation, lymphocyte differentiation and lymphocyte activation. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse ICAM-1 (intercellular adhesion molecule); CD54 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse ICAM-1 (intercellular adhesion molecule); CD54 |
| Comments |
Animals were immunized with ICAM-1 transfected FT16.I1 cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody blocks the binding of lymphocyte function antigen 1 (LFA-1, CD11a, CD18) to ICAM-1. The antiboy is useful for studies of inflammation, lymphocyte differentiation and lymphocyte activation. It can be used for purification of ICAM-1 and for staining of cell surface ICAM-1 by flow cytometry. |
| Complete Growth Medium |
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
AA Brian |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Kuhlman P, et al. The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation. J. Immunol. 146: 1773-1782, 1991. PubMed: 1672331
|
