| 產(chǎn)品名稱(chēng) |
Bet 1 |
| 商品貨號(hào) |
B164026 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse IgM (Igh-6.5 allotype) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse IgM (Igh-6.5 allotype) |
| Comments |
Animals were immunized with TEPC 183 myeloma protein. Spleen cells were fused with NS-1 myeloma cells. The antibody is not cytotoxic and does not bind to protein A. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 1 to 2 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
JT Kung |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Kung JT, et al. A mouse IgM allotypic determinant (Igh-6.5) recognized by a monoclonal rat antibody. J. Immunol. 127: 873-876, 1981. PubMed: 6167630
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