| 產品名稱 |
BF-11 |
| 商品貨號 |
B164030 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody binds alpha, beta and asialo forms of human erythropoietin. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human erythropoietin |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human erythropoietin |
| Comments |
Animals were immunized with human urinary erythropoietin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds alpha, beta and asialo forms of human erythropoietin. The antibody has been given the name Epoclonalan. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a |
| Name of Depositor |
University Patents, Inc. |
| U.S. Patent Number |
|
| References |
Weiss TL, et al. Characterization of a monoclonal antibody to human erythropoietin. Proc. Natl. Acad. Sci. USA 79: 5465-5469, 1982. PubMed: 6957875
Goldwasser E, et al. Monoclonal anti-erythropoietin. US Patent 4,558,005 dated Dec 10 1985
|
