Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
normal
Age
embryo
Strain
C57BL/6
Applications
This line was derived from BLK CL.4 (ATCC TIB-81) by transformation with SV40.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from BLK CL.4 (ATCC TIB-81) by transformation with SV40.
Tumorigenic
Yes
Effects
Yes, in ATxFL mice but not in normal C57BL/6 mice
Comments
This line was derived from BLK CL.4 (ATCC TIB-81) by transformation with SV40.
The cells will grow in 0.3% agarose.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove old medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor
P Patek, J Collins, M Cohn
Deposited As
Mus musculus
References
Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934
