| 產品名稱 |
BUD-8 |
| 商品貨號 |
B164055 |
| Organism |
Homo sapiens, human |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
56 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Applications |
This line is highly sensitive to interferon and can be used in assays of interferon activity. |
| Clinical Data |
female Caucasian 56 years |
| Comments |
This line is highly sensitive to interferon and can be used in assays of interferon activity. The line is capable of at least 8 population doublings after thawing. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Twice per week Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| STR Profile |
Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 11,12 D5S818: 10,12 D7S820: 8,10 THO1: 9 TPOX: 11 vWA: 16,17 |
| Isoenzymes |
G6PD, B |
| Name of Depositor |
BW Uhlendorf |
| Deposited As |
Homo sapiens |
| References |
Interferon bioassay methods and the development of standard procedures: a critique and analysis of current observations. Rockville, MD: Tissue Culture Association; 1974.
Grossberg SE, et al. Interferon standards: a memorandum. J. Biol. Stand. 7: 383-395, 1979. PubMed: 536379
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