| 產品名稱 |
BVD2-21C11.3 |
| 商品貨號 |
B164056 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? Lewis (B cell); BALB/c (myeloma) |
| Applications |
The antibody binds to and blocks the activity of human GM-CSF. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified human GM-CSF. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human granulocyte - macrophage colony stimulating factor (GM-CSF) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human granulocyte - macrophage colony stimulating factor (GM-CSF) |
| Comments |
Animals were immunized with purified human GM-CSF. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody binds to and blocks the activity of human GM-CSF. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 0.05 mM 2-mercaptoethanol
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5%. |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a |
| Name of Depositor |
Schering Corp. |
| Deposited As |
rat (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Abrams JS, VanDyke RE. Immunochemical assay for human granulocyte-macrophage colony stimulating factor. US Patent 5,070,013 dated Dec 3 1991
|
