波多野结衣毛片-乳色吐息在线观看-国产伦子伦对白视频-性做久久久-狠狠干2019-黄色裸体片-美女无遮挡免费网站-国产91熟女高潮一区二区-懂色av蜜臀av粉嫩av分享-小h片在线观看-台湾佬在线-日韩激情在线播放-欧日韩不卡在线视频-波多野结衣中文字幕一区-天天操夜夜草

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > BW5147.3
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
BW5147.3
BW5147.3
規格:
貨期:
編號:B164059
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 BW5147.3
商品貨號 B164059
Organism Mus musculus, mouse
Tissue thymus
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain AKR/J
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a clone of the BW5147 cell line which was established from a spontaneous AKR/J thymoma.
Antigen Expression
H-2k; Thy-1.1
Comments

The cells are HAT resistant but sensitive to 0.001 mM cortisol and to 25 mcg/mL PHA.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL. Do not allow the cell concentration to exceed 2 x 10cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Name of Depositor R Hyman, P Ralph
Deposited As Mus musculus
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Kohler G, et al. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7: 758-761, 1977. PubMed: 563329

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 婷婷色影院 | 国产精品视频一区二区三区不卡 | 四虎成人影视 | 亚洲精品日本 | 午夜大片网 | 色哟哟官网 | 久久久久久久久久国产精品 | 欧美特黄一级大片 | 91一区二区 | 中文字幕一区二区在线视频 | 超碰蜜桃 | 欧美精品久久久久久久多人混战 | 重口另类| 手机在线看a | 亚洲第一伊人 | 丰满少妇被猛烈进入无码 | 免费国产小视频 | 日韩一区二区视频在线 | 91cn.com| 性感美女被草 | 亚洲精品1| 影音先锋久久久久av综合网成人 | 亚洲精品美女在线观看 | 成人毛片在线播放 | 欧美在线精品一区二区三区 | 69超碰| 丁香伊人网 | 黄色网址av | 少妇一区二区三区四区 | 国产乱淫av一区二区三区 | 亚洲高清免费视频 | 一区二区免费在线观看 | 黄色一节片 | 少妇一级淫片免费观看 | 国产无遮挡aaa片爽爽 | 日韩在线精品 | 午夜精品久久久久久久99 | 无码人妻精品一区二区三区不卡 | 先锋av资源网 | 美女视频国产 | 五月婷婷影院 | 欧美人性生活视频 | 欧美aⅴ在线 | 欧美一区二区三区免费在线观看 | 亚洲国产日本 | 一区二区三区视频免费观看 | www在线免费观看 | 国产免费不卡视频 | 国产黄色在线 | 两个人看的www视频免费完整版 | 精品一区二区久久久久久久网站 | 国产精品免费看久久久无码 | 欧美日韩在线一区二区三区 | 嫩草av91| 精品久久久久久亚洲综合网站 | 三上悠亚亚洲一区 | 亚洲一区区 | 成人免费视频一区二区 | 欧美精品1 | 欧美精品一级片 | 手机看片一区二区三区 | 国产精品高清网站 | 白浆四溢 | 男男巨肉啪啪动漫3d | 久久久久国产一区二区 | 在线看黄色av| 在线激情网 | 国产系列精品av | 50一60岁老妇女毛片 | 97在线播放 | 国产精品sm调教免费专区 | 国内精品久久久久久久久久 | 四虎视频国产精品免费 | 欧美理伦 | 另类av小说| 国产馆av| 泽村玲子av | 奇米四色网 | 日韩视频播放 | 宅男午夜影院 | 日本理论片 | 合欢视频在线观看 | 日本黄色特级片 | 看a网站 | 日韩欧美高清视频 | 日本少妇激情舌吻 | 精品午夜一区二区三区 | 黄色大片在线 | 国产精品女人久久久 | 一级片中文字幕 | 日日夜夜中文字幕 | 午夜在线观看av | 国产小视频在线观看 | 北岛玲在线 | 亚洲欧美校园春色 | 国产玖玖 | 亚洲aⅴ | 性久久久久 | 久草视频免费在线观看 |