| 產品名稱 |
BZR |
| 商品貨號 |
B164064 |
| Organism |
Homo sapiens, human |
| Tissue |
lung, bronchus |
| Cell Type |
epithelial virus transformed |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinogen |
| Applications |
The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
This line was derived from BEAS-2B cells (see ATCC CRL-9609) by infection with the recombinant retrovirus Zip-neo-v-Ha-ras (constructed by recombining the pZipNeoSV(X) with a fragment containing the v-Ha-ras oncogene. Cells were selected in medium containing G418. |
| Tumorigenic |
Yes |
| Effects |
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments |
The line is reported to be highly tumoriogenic, and to stain positively for keratins and SV40 T antigen.
|
| Complete Growth Medium |
The base medium for this cell line (BEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: BEGM, Kit Catalog No. CC-3170. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with the BEGM kit.
Note: Do not filter complete medium.
|
| Subculturing |
Remove medium, add 0.25%trypsin-0.53mM EDTA with 0.5% PVP (polyvinylpirrolidone) solution and allow the culture to sit at room temperature until the cells begin to detach (usually 5 to 10 minutes). Add fresh medium, wash by centrifugation, aspirate and dispense into new flasks. Inoculate new flask at 1500 to 3000 cells per sq. cm. The flasks used should be precoated with with a mixture of 0.01 mg/mL fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin (see above reference). The cells should be subcultured before reaching confluence since confluent cultures rapidly undergo squamous terminal differentiation.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Leibovitz?s L-15 medium with 2 mM L-glutamine supplemented with 10 mM HEPES, 1% PVP, 10% fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: XY CSF1PO: 9, 12 D13S317: 13 D16S539: 12 D5S818: 12,13 D7S820: 10, 13 THO1: 7, 9.3 TPOX: 6, 11 vWA: 17, 18 |
| Name of Depositor |
The United States of America |
| U.S. Patent Number |
|
| References |
Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989
Lechner JF, LaVeck MA. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Culture Methods 9: 43-48, 1985.
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