| 產品名稱 |
CAB 117-12D10 [12D10] |
| 商品貨號 |
B164107 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma:lymphoblast B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
adult |
| Gender |
female |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The 12D10 monoclonal antibody reacts specifically with both factor VII and factor VIIa antigen. It is specific to amino acids 195-208 on the factor VII/VIIa molecule. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified human factor VII (factor VII) isolated from pooled human plasma. Spleen cells were fused with Sp2/0 mouse myeloma cells. |
| Clinical Data |
female |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against factors VII and VIIa (VII/VIIa) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against factors VII and VIIa (VII/VIIa) |
| Comments |
Animals were immunized with purified human factor VII (factor VII) isolated from pooled human plasma. Spleen cells were fused with Sp2/0 mouse myeloma cells. The 12D10 monoclonal antibody reacts specifically with both factor VII and factor VIIa antigen. It is specific to amino acids 195-208 on the factor VII/VIIa molecule. |
| Complete Growth Medium |
A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%
A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Two to three times weekly |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 kappa |
| Name of Depositor |
Corvas, Inc. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Soule HR, Brunck TK. Hypridoma and monoclonal antibody which inhibits blood coagulation tissue factor/factor VIIa complex. US Patent 5,506,134 dated Apr 9 1996
Biemond BJ, et al. Complete inhibition of endotoxin-induced coagulation activation in chimpanzees with a monoclonal Fab fragment against factor VII/VIIa. Thromb. Haemostasis 73: 223-230, 1995. PubMed: 7792734
|
