| 產(chǎn)品名稱 | Caov-4 |
|---|---|
| 商品貨號 | B164118 |
| Organism | Homo sapiens, human |
| Tissue | ovary; derived from metastatic site: subserosa of fallopian tube |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma |
| Age | 45 years |
| Gender | female |
| Ethnicity | Caucasian |
| Karyotype | (P5) hypotriploid and hypertetraploid with abnormalities including breaks, dicentrics, minute chromatin bodies, fragmentations, secondary constrictions and large subtelocentric and submetacentric markers |
| Images |  |
| Clinical Data | 45 years Caucasian female |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing | Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation | Freeze medium: complete growth medium supplemented with an additional 40% fetal bovine serum and 10% (v/v) DMSO |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 100% |
| Isoenzymes | AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1-2 PGM3, 2 |
| Name of Depositor | J Fogh |
| Deposited As | Homo sapiens |
| References | Yaginuma Y, et al. Analysis of the Rb gene and cyclin-dependent kinase 4 inhibitor genes (p16INK4 and p15INK4B) in human ovarian carcinoma cell lines. Exp. Cell Res. 233: 233-239, 1997. PubMed: 9194486 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
