| 產品名稱 |
CC 49 [CC-49, CC49] |
| 商品貨號 |
B164125 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody binds to TAG-72 with an affinity constant of 20 X 10 exp9 liter/mol. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with affinity purified TAG-72 from LS 174T cells (see ATCC CL-188). Spleen cells were fused with NS-1 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against TAG-72 human tumor associated glycoprotein |
| Cellular Products |
immunoglobulin; monoclonal antibody; against TAG-72 human tumor associated glycoprotein |
| Comments |
Animals were immunized with affinity purified TAG-72 from LS 174T cells (see ATCC CL-188). Spleen cells were fused with NS-1 mouse myeloma cells. The antibody binds to TAG-72 with an affinity constant of 20 X 10 exp9 liter/mol. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; heat-inactivated fetal bovine serum, 15%
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
mouse IgG1 kappa |
| Name of Depositor |
The United States of America |
| U.S. Patent Number |
|
| References |
Schlom J, Colcher D. Second generation monoclonal antibodies having binding specificity to TAG-72 and human carcinomas and methods for employing the same. US Patent 5,512,443 dated Apr 30 1996
|
