| 產品名稱 |
CC30 |
| 商品貨號 |
B164131 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the 50000 dalton bovine CD4 molecule. |
| Derivation |
Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine CD4 (BoCD4) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine CD4 (BoCD4) |
| Comments |
Animals were immunized with bovine lymphocytes and thymocytes. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for the 50000 dalton bovine CD4 molecule. The CD4 antigen is expressed on T cells that are CD2 positive and bear the gamma/delta T cell receptor. This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992. |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
CJ Howard |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Oldham G, et al. In vivo role of lymphocyte subpopulations in the control of virus excretion and mucosal antibody responses of cattle infected with rotavirus. J. Virol. 67: 5012-5019, 1993. PubMed: 8392626
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