| 產品名稱 |
CC39 |
| 商品貨號 |
B164133 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for bovine WC1. It is similar in specificity to CC15 (see ATCC HB-265), but appears to bind to a different epitope. |
| Derivation |
Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine WC1 (BoWC1) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine WC1 (BoWC1) |
| Comments |
Animals were immunized with bovine thymocytes. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for bovine WC1. It is similar in specificity to CC15 (see ATCC HB-265), but appears to bind to a different epitope. This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992. |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
CJ Howard |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. PubMed: 1708546
. . Vet. Immunol. Immunopathol. 32: 1993..
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