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CC42

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編號:B164134

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產品名稱	CC42
商品貨號	B164134
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody is specific for the bovine CD2 T cell antigen.
Derivation	Spleen cells were fused with NS1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against bovine CD2 (BoCD2)
Cellular Products	immunoglobulin; monoclonal antibody; against bovine CD2 (BoCD2)
Comments	Animals were immunized with bovine thymocytes. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for the bovine CD2 T cell antigen. This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992.
Complete Growth Medium	Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation	Culture medium, 95%; DMSO, 5%
Isotype	IgG1
Name of Depositor	CJ Howard
Deposited As	mouse (B cell); mouse (myeloma)
References	Howard CJ, et al. In vivo depletion of BoT4 (CD4) and of non-T4/T8 lymphocyte subsets in cattle with monoclonal antibodies. Eur. J. Immunol. 19: 757-764, 1989. PubMed: 2567247 Seidler RJ, et al. L-selectin expression differentiates T cells isolated from different lymphoid tissues in cattle but does not correlate with memory. Immunology 77: 228-234, 1992. PubMed: 1385313 Oldham G, Howard CJ. Suppression of bovine lymphocyte responses to mitogens following in vivo and in vitro treatment with dexamethasone. Vet. Immunol. Immunopathol. 30: 161-177, 1992. PubMed: 1534431 Howard CJ, et al. Immunity to bovine virus diarrhoea virus in calves: the role of different T-cell subpopulations analysed by specific depletion in vivo with monoclonal antibodies. Vet. Immunol. Immunopathol. 32: 303-314, 1992. PubMed: 1321529

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