| 產(chǎn)品名稱 |
CCD-1097Sk |
| 商品貨號 |
B164168 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma |
| Age |
31 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Applications |
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma. |
| Derivation |
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma. |
| Clinical Data |
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma. female Caucasian 31 years |
| Comments |
The line was established from normal skin from the breast of a patient undergoing biopsy of a grade III infiltrating ductal carcinoma. Cells from the current passage (1) have experienced about 3 population doublings beyond the biopsy material and are capable of at least 8 additional population doublings. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Twice per week Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Passage History |
Cells from the current passage (1) have experienced about 3 population doublings beyond the biopsy material and are capable of at least 8 additional population doublings. |
