| 產(chǎn)品名稱 |
CCD-25Sk |
| 商品貨號(hào) |
B164201 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Glioma |
| Age |
7 years |
| Gender |
male |
| Applications |
The line was derived from normal skin of a patient that died from a high grade glioma. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was derived from normal skin of a patient that died from a high grade glioma. |
| Clinical Data |
The line was derived from normal skin of a patient that died from a high grade glioma. male |
| Comments |
The line was derived from normal skin of a patient that died from a high grade glioma. This line is capable of at least 8 population doublings from the thawed ampule. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Let the culture sit at room temperature or at 37°C until cells detach (about 10 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation |
Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10,14 D16S539: 9,11 D5S818: 12 D7S820: 8,11 THO1: 6,7 TPOX: 11,12 vWA: 17,18 |
