| 產(chǎn)品名稱 |
CF4-C4 |
| 商品貨號 |
B164235 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Antigen Expression |
H-2d |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen),H-2d |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen) |
| Comments |
Mice were immunized with naked Salmonella minnesota to which had been adsorbed the neutral glycolipid fraction from human Type A meconium. Spleen cells were fused with NS-1 myeloma cells. The antibody recognizes all Lewis a molecules regardless of the internal linkage of the carbohydrate chain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
RPMI 1640 medium, 85%; fetal bovine serum, 15%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
WW Young |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Young WW Jr., et al. Characterization of monoclonal antibodies specific for the Lewis a human blood group determinant. J. Biol. Chem. 258: 4890-4894, 1983. PubMed: 6833281
|
