| 產(chǎn)品名稱 |
CH1 |
| 商品貨號(hào) |
B164241 |
| Organism |
Mus musculus, mouse |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
B10.H-2aH-4bp/Wts(2a4b) |
| Applications |
transfection host |
| Derivation |
This line was established in tissue culture from the CH1 B cell lymphoma. |
| Antigen Expression |
I-Ak; I-Ek |
| Genes Expressed |
immunoglobulin (surface); against E. coli,I-Ak; I-Ek |
| Cellular Products |
immunoglobulin (surface); against E. coli |
| Comments |
This line was established in tissue culture from the CH1 B cell lymphoma. The cells produce, but do not secrete, immunoglobulin. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml and maintain between 3 x 10 exp5 and 8 x 10 exp5 cells/ml. |
| Isotype |
IgM; lambda light chain |
| Name of Depositor |
G Haughton |
| Deposited As |
Mus musculus |
| References |
Lanier LL, et al. Transplantable B-cell lymphomas in B10. H-2aH-4bp/Wts mice. Immunogenetics 16: 367-371, 1982. PubMed: 6983495
Lanier LL, et al. Novel type of murine B-cell lymphoma. Nature 271: 554-555, 1978. PubMed: 304532
J. Immunol. 121: 2353, 1978.
|
