| 產品名稱 |
CHL/IU [CHL-11] |
| 商品貨號 |
B164247 |
| Organism |
Cricetulus griseus, hamster, Chinese |
| Tissue |
lung |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
newborn |
| Gender |
female |
| Applications |
The line is useful for screening chemical for ability to induce chromosomal aberrations. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 25 |
| Derivation |
This line was derived from the CHL line established by Utakoji in 1970. |
| Complete Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; bovine calf serum, 10%
|
| Subculturing |
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week |
| Cryopreservation |
Complete growth medium supplemented with 5% DMSO |
| Culture Conditions |
Temperature: 37°C |
| Population Doubling Time |
15 hrs |
| Name of Depositor |
M Ishidate |
| Deposited As |
Cricetulus griseus |
| Year of Origin |
1970 |
| References |
Data book of chromosomal aberration test in vitro. New York: Elsevier; 1988.
Ishidate M Jr., Odashima S. Chromosome tests with 134 compounds on Chinese hamster cells in vitro-- a screening for chemical carcinogens. Mutat. Res. 48: 337-354, 1977. PubMed: 876270
Koyama H, et al. A new cell line derived from newborn Chinese hamster lung tissue. Gann 61: 161-167, 1970. PubMed: 5452405
|
