| 產(chǎn)品名稱 |
CHL-1 |
| 商品貨號(hào) |
B164248 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Cell Type |
melanoma |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
melanoma |
| Applications |
Transfection host derived from RPMI 7932 that can grow to a higher density, namely 5 x 107 cells/mL. |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
This is a derivative of the RPMI 7932 human melanoma cell line (Bowes). |
| Genes Expressed |
human tissue plasminogen activator (t-PA) |
| Cellular Products |
human tissue plasminogen activator (t-PA) |
| Comments |
This cell line produces about 0.1 mU of t-PA per cell per day. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Add fresh medium at the time of subculture |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 8,11 D16S539: 13 D5S818: 11,12 D7S820: 11,13 THO1: 9,9.3 TPOX: 8 vWA: 17 |
| Name of Depositor |
Berlex Laboratories, Inc. |
| U.S. Patent Number |
|
| References |
Cashion L, et al. Transfected cells containing plasmids having genes oriented in opposing directions and methods of using. US Patent 5,017,478 dated May 21 1991
|
