| Applications |
The antibody reacts with canine intercellular adhesion molecule 1 (ICAM-1). Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells. |
| Comments |
Animals were immunized with canine endothelial cells that had been stimulated with lipopolysaccharide (LPS). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with canine intercellular adhesion molecule 1 (ICAM-1). Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells. |
| References |
Smith CW, et al. Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. J. Clin. Invest. 88: 1216-1223, 1991. PubMed: 1680878
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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